Zika

Animal Models

  • BALB/c, A129, and AG129 mice
  • Rhesus and cynomolgus macaques
  • Pregnant macaques

Study Types

  • Vaccine testing
  • Therapeutics testing
  • PK/safety testing
  • Pathogenesis

In vitro Services

  • Sample processing
  • RT-PCR viral load testing
  • Binding antibody ELISA and PRNT
  • Flow-based characterization of PBMC populations
  • Functional T cell assays

Representative Publications

Impact of prior Dengue immunity on Zika vaccine protection in rhesus macaques and mice.
Larocca RA, Abbink P, Ventura JD, Chandrashekar A, Mercado N, Li Z, Borducchi E, De La Barrera RA, Eckels KH, Modjarrad K, Busch MP, Michael NL, Barouch DH.
PLoS Pathog. 2021 Jun 25;17(6):e1009673. doi: 10.1371/journal.ppat.1009673. eCollection 2021 Jun.
PMID:34170962 Free PMC article.

Development of a potent Zika virus vaccine using self-amplifying messenger

Valiant WG, Mattapallil MJ, Higgs S, Huang YS, Vanlandingham DL, Lewis MG, Mattapallil JJ. Simultaneous Coinfection of Macaques with Zika and Dengue Viruses Does not Enhance Acute Plasma Viremia but Leads to Activation of Monocyte Subsets and Biphasic Release of Pro-inflammatory Cytokines. Sci Rep. 2019 May 27;9(1):7877.

Valiant WG, Huang YS, Vanlandingham DL, Higgs S, Lewis MG, Mattapallil JJ. Zika convalescent macaques display delayed induction of anamnestic cross-neutralizing antibody responses after dengue infection. Emerg Microbes Infect. 2018 Jul 13;7(1):130.

Martinot AJ, Abbink P, Afacan O, Prohl AK, Bronson R, Hecht JL, Borducchi EN, Larocca RA, Peterson RL, Rinaldi W, Ferguson M, Didier PJ, Weiss D, Lewis MG, De La Barrera RA, Yang E, Warfield SK, Barouch DH. Fetal Neuropathology in Zika Virus-Infected Pregnant Female Rhesus Monkeys. Cell. 2018 May 17;173(5):1111-1122.e10

Medina LO, To A, Lieberman MM, Wong TAS, Namekar M, Nakano E, Andersen H, Yalley-Ogunro J, Greenhouse J, Higgs S, Huang YS, Vanlandingham DL, Horton JS, Clements DE, Lehrer AT. A Recombinant Subunit Based Zika Virus Vaccine Is Efficacious in Non-human Primates. Front Immunol. 2018 Nov 8;9:2464. doi: 10.3389/fimmu.2018.02464. eCollection 2018.

Abbink P, Larocca RA, Visitsunthorn K, Boyd M, De La Barrera RA, Gromowski GD, Kirilova M, Peterson R, Li Z, Nanayakkara O, Nityanandam R, Mercado NB, Borducchi EN, Chandrashekar A, Jetton D, Mojta S, Gandhi P, LeSuer J, Khatiwada S, Lewis MG, Modjarrad K, Jarman RG, Eckels KH, Thomas SJ, Michael NL, Barouch DH. Durability and correlates of vaccine protection against Zika virus in rhesus monkeys. Sci Transl Med. 2017 Dec 13;9(420).

Pardi N, Hogan MJ, Pelc RS, Muramatsu H, Andersen H, DeMaso CR, Dowd KA, Sutherland LL, Scearce RM, Parks R, Wagner W, Granados A, Greenhouse J, Walker M, Willis E, Yu JS, McGee CE, Sempowski GD, Mui BL, Tam YK, Huang YJ, Vanlandingham D, Holmes VM, Balachandran H, Sahu S, Lifton M, Higgs S, Hensley SE, Madden TD, Hope MJ, Karikó K, Santra S, Graham BS, Lewis MG, Pierson TC, Haynes BF, Weissman D. Zika virus protection by a single low-dose nucleoside-modified mRNA vaccination. Nature. 2017 Mar 9;543(7644):248-251.

Aid M, Abbink P, Larocca RA, Boyd M, Nityanandam R, Nanayakkara O, Martinot AJ, Moseley ET, Blass E, Borducchi EN, Chandrashekar A, Brinkman AL, Molloy K, Jetton D, Tartaglia LJ, Liu J, Best K, Perelson AS, De La Barrera RA, Lewis MG, Barouch DH. Zika Virus Persistence in the Central Nervous System and Lymph Nodes of Rhesus Monkeys. Cell. 2017 May 4;169(4):610-620.e14.

Abbink P, Larocca RA, Visitsunthorn K, Boyd M, De La Barrera RA, Gromowski GD, Kirilova M, Peterson R, Li Z, Nanayakkara O, Nityanandam R, Mercado NB, Borducchi EN, Chandrashekar A, Jetton D, Mojta S, Gandhi P, LeSuer J, Khatiwada S, Lewis MG, Modjarrad K, Jarman RG, Eckels KH, Thomas SJ, Michael NL, Barouch DH. Durability and correlates of vaccine protection against Zika virus in rhesus monkeys. Sci Transl Med. 2017 Dec 13;9(420).

George J, Valiant WG, Mattapallil MJ, Walker M, Huang YS, Vanlandingham DL, Misamore J, Greenhouse J, Weiss DE, Verthelyi D, Higgs S, Andersen H, Lewis MG, Mattapallil JJ. Prior Exposure to Zika Virus Significantly Enhances Peak Dengue-2 Viremia in Rhesus Macaques. Sci Rep. 2017 Sep 5;7(1):10498.

Dowd, KA, Sung-Youl Ko, Kaitlyn M. Morabito, Eun Sung Yang, Rebecca S. Pelc, Christina R. DeMaso, Leda R. Castilho, Peter Abbink, Michael Boyd, RamyaNityanandam, David N. Gordon, John Gallagher, Xuejun Chen, John-Paul Todd, YaroslavTsybovsky, Audray Harris, Yan-Jang S. Huang, Stephen Higgs, Dana L. Vanlandingham, Hanne Andersen, Mark G. Lewis, Rafael De La Barrera, Kenneth H. Eckels, Richard G. Jarman, Martha C. Nason, Dan H. Barouch, Mario Roederer, Wing-Pui Kong, John R. Mascola, Theodore C. Pierson, Barney S. Graham. Rapid Development of a Zika Virus Vaccine. Science 2016; September 22.10.1126/Science.aai3197.

Osuna, CE, So-Yon Lim, Jacob D. Estes, Claire Deleage, Erwing Fabian Cardozo Ojeda, Lucas Schroeder, Katharine Best, Ma Lou, Peter T. Hraber, Hanne Andersen Elyard, Scott Huang, Stephen Higgs, Alan Perelson, David Safronetz, Mark G. Lewis and James B. Whitney. Viral Dynamics and Shedding in Zika Virus Infected Macaques. Nature Medicine. October 3, 2016. DOI: 10.1038/nm.4206.

Abbink P, Larocca RA, De La Barrera RA, Bricault CA, Moseley ET, Boyd M, Kirilova M, Li Z, Ng’ang’a D, Nanayakkara O, Nityanandam R, Mercado NB, Borducchi EN, Agarwal A, Brinkman AL, Cabral C, Chandrashekar A, Giglio PB, Jetton D, Jimenez J, Lee BC, Mojta S, Molloy K, Shetty M, Neubauer GH, Stephenson KE, Peron JP, Zanotto PM, Misamore J, Finneyfrock B, Lewis MG, Alter G, Modjarrad K, Jarman RG, Eckels KH, Michael NL, Thomas SJ, Barouch DH. Protective efficacy of multiple vaccine platforms against Zika virus challenge in rhesus monkeys. Science. 2016 Sep 9;353(6304):1129-32. doi: 10.1126/science.aah6157. PubMed PMID: 27492477.