Influenza

Animal Models

  • BALB/c mice
  • Ferrets
  • Various NHPs

Study Types

  • Vaccine testing
  • Therapeutics testing
  • PK/safety testing
  •  Dose-ranging studies
  • Transfusions
  • ABSL-3 (including Select Agent) and enhanced BSL-2 requiring studies

In vitro Services

  • Virus stock generation in embryonated chicken eggs
  • Various H1N1, H2N2, H3N2, H5N1, H7N3, H7N7, H7N9, H9N2 and B virus strains available
  • Sample processing
  • ELISA, HAI, ELLA and MN assays
  • Infectious and qRT-PCR viral load testing
  • Ferret cytokine/chemokine mRNA levels
  • ELISPOT assay

Representative Publications

Influenza Antigens NP and M2 Confer Cross Protection to BALB/c Mice against Lethal Challenge with H1N1, Pandemic H1N1 or H5N1 Influenza A Viruses. Mytle N, Leyrer S, Inglefield JR, Harris AM, Hickey TE, Minang J, Lu H, Ma Z, Andersen H, Grubaugh ND, Guina T, Skiadopoulos MH, Lacy MJ. Viruses. 2021 Aug 27;13(9):1708. doi: 10.3390/v13091708. PMID: 34578289

Zachary R. Sia, Xuedan He, Ali Zhang, Jann C. Ang, Shuai Shao, Amal Seffouh, Wei-Chiao Huang, Michael R. D’Agostino, Amir Teimouri Dereshgi, Sambhara Suryaprakash, Joaquin Ortega, Hanne Andersen, Matthew S. Miller, Bruce A. Davidson, Jonathan F. Lovell. A liposome-displayed hemagglutinin vaccine platform protects mice and ferrets from heterologous influenza virus challenge. Proceedings of the National Academy of Sciences Jun 2021, 118 (22) e2025759118; DOI: 10.1073/pnas.2025759118.

Masaru Kanekiyo, M. Gordon Joyce, Rebecca A. Gillespie, John R. Gallagher, Sarah F. Andrews, Hadi M. Yassine, Adam K. Wheatley, Brian E. Fisher, David R. Ambrozak, Adrian Creanga, Kwanyee Leung, Eun Sung Yang, Seyhan Boyoglu-Barnum, Ivelin S. Georgiev, Yaroslav Tsybovsky, Madhu S. Prabhakaran, Hanne Andersen, Wing-Pui Kong, Ulrich Baxa, Kathryn L. Zephir, Julie E. Ledgerwood, Richard A. Koup, Peter D. Kwong, Audray K. Harris, Adrian B. McDermott, John R. Mascola & Barney S. Graham. Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses. Nature Immunology, volume 20, pages 362–372 (2019).

DiNapoli, J. M., Nayak, B., Yang L., Finneyfrock B.W., Cook A., Andersen H., Torres-Velez F., Murphy B., Samal S. K., Collins P. L., and Bukreyev A. Newcastle disease virus-vectored Vaccines expressing the hemagglutinin or neuraminidase protein of H5N1 highly pathogenic avian influenza virus protect against virus challenge in monkeys. J. Virol. 2009 Nov. 18

Wei CJ, Boyington JC, McTamney PM, Kong WP, Pearce MB, Xu L, Andersen H, Rao S, Tumpey TM, Yang ZY, Nabel GJ. Induction of Broadly Neutralizing H1N1 Influenza Antibodies by Vaccination. Science. 2010 Jul 15. PMID: 20647428.

Yassine HM, Boyington JC, McTamney PM, Wei C-J, Kanekiyo M, Kong W-P, Gallagher JR, Wang L, Zhang Y, Joyce MG, Lingwood D, Moin SM, Andersen H, Okuno Y, Rao SS, Harris AK, Kwong PD, Mascola JR, Nabel GJ, and BS Graham. Hemagglutinin-stem nanoparticles generate heterosubtypic influenza protection. Nature Medicine (2015). 24 August. doi:10.1038/nm.3927.